Exosomes Derived from Brain Metastatic Breast Cancer Cells Destroy the Blood-Brain Barrier by Carrying lncRNA GS1-600G8.5

Figure 3

Exosomes derived from high brain metastatic cells destroys BBB permeability. (a) Transmission electron microscopy appearance of exosomes. (b) Western blot measurement of exosome markers in cells and exosomes. (c) Exosomes derived from MDA231 low metastatic breast cancer cells and MDABR3 high brain metastatic breast cancer cells were labeled with PKH26 and added to the BBB model. Representative pictures of BMECs internalizing the exosomes (magnification ×200). (d) Exosomes were added to the BBB system and the TEER value was monitored after 24 h. NC: negative control (BBB system without exosome treatment). (e) Permeability was detected after exosomes incubated BBB system for 24 h. (f) Exosomes were added to the BBB model and incubated for 24 h, MDA231 (PFG-labeled) cells were added to the BBB model, and the number of breast cancer cells crossing the BBB were counted after 48 h. (Magnification ×200, one-way ANOVA, , ).

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