Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor Alpha/B-Cell Lymphoma 6 Pathway

<div>HIF-1<i>α</i>/BCL-6 upregulation reversed the effect of PKM2 knockdown on gastric carcinoma EMT. MKN-45 and SGC-7901 cells were transfected with control siRNA, PKM2 siRNA, PKM2 siRNA+pcDNA3.1, PKM2 siRNA+pcDNA3.1-HIF-1<i>α</i>, or PKM2 siRNA+pcDNA3.1-BCL-6. Western blot assays determined the protein expression of HIF-1<i>α</i> and BCL-6 in (a) MKN-45 and (b) SGC-7901 cells. The mRNA levels of EMT markers in (c) MKN-45 and (d) SGC-7901 cells were measured using qRT-PCR. 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BioMed Research International

fig3

Figure 3

Figure 3: Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor Alpha/B-Cell Lymphoma 6 Pathway 

Figure 3 | Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor Alpha/B-Cell Lymphoma 6 Pathway 