Association between Vitamin D Receptor Single-Nucleotide Polymorphisms and Colorectal Cancer in the Thai Population: A Case-Control Study

Figure 1

Representative RFLP patterns of each VDR polymorphism of the 5 VDR-SNPs and example of DNA sequencing results of three PCR products of three cases with different Tru9I genotypes. (a) FokI (rs228570 C>T): product sizes were 266 bp for the C allele and 205 and 61 bp for the T allele, (b) BsmI (rs1544410 A>G): product sizes were 357 bp for the A allele and 254 and 103 bp for the G allele, (c) ApaI (rs7975232 G>T): product sizes were 340 bp for the T allele and 229 and 111 bp for the G allele, (d) TaqI (rs731236 C>T): product sizes were 340 bp for the T allele and 191 and 149 bp for the C allele, (e) Tru9I (rs757343 G>A): product sizes were 357 bp for the G allele and 26 and 93 bp for the A allele, and (f) DNA sequencing results of the PCR products with Tru9I (rs757343 G>A) genotypes GG, GA, and AA. The arrow indicates the polymorphism; M: a molecular marker of the 100 bp ladder.

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