Comparison of Primer-Probe Sets among Different Master Mixes for Laboratory Screening of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)

Table 5

The results of Invitrogen™ SuperScript™ III One-Step RT-PCR System with three primer-probe sets (IDT, Phu Sa, and TIB-Molbiol).

Dilution	Copies/reaction	TIB-Molbiol; values			IDT; values			Phu Sa; values
		Day 1	Day 2	Day 3	Day 1	Day 2	Day 3	Day 1	Day 2	Day 3
1 : 10^8	96	34.23	34.51	34.81	35.07	34.91	35.03	34.34	35.21	35.31
		34.16	34.66	34.6	35.15	33.94	34.34	35.03	34.87	34.62
		34.09	34.09	34.83	35.12	34.82	34.21	34.24	34.86	35.32
1 : 10^8½	48	36.31	35.72	36.16	36.53	35.44	35.84	35.84	36.36	36.12
		35.58	35.58	36.34	36.64	36.41	36.34	36.81	36.53	35.74
		35.69	36.00	36.14	36.58	35.71	36.66	36.71	36.34	36.83
1 : 10^9	9.6	37.09	37.83	37.16	36.94	37.34	37.91	37.34	38.32	38.31
		37.23	37.09	37.71	38.03	38.07	38.15	37.86	38.21	37.87
		37.51	37.66	37.6	38.1	37.82	37.19	38.03	37.62	37.24
1 : 10^9½	4.8	38.59	38.59	38.66	39.62	39.32	38.82	38.82	39.72	38.74
		39.33	38.63	39.1	38.42	38.71	39.51	39.77	39.59	39.52
		39.16	39.01	39.31	39.46	39.41	39.55	39.36	39.12	39.37
Negative control		-	-	-	-	-	-	-	-	-
Positive control		24.27	25.91	22.81	28.40	28.38	26.72	20.81	30.56	29.39

“-”: undetected.