Germacrone Inhibits Cell Proliferation and Induces Apoptosis in Human Esophageal Squamous Cell Carcinoma Cells

Figure 2

Germacrone inhibits ESCC cell migration. (a, b) Wound healing assays were performed on Eca109 and EC9706 cells. After the creation of the scratch wounds, cells were treated with 0, 5, 10, and 15 μg/mL of germacrone and photographed at 0, 12, and 24 h. (c, d) The relative wound surface area was calculated using ImageJ software. (e)–(h) Western blot analysis of the relative expression of MMP-9 protein in Eca109 and EC9706 cells treated with germacrone for 24 h. Data were presented as means ± SD (n = 3). , vs. control (0 μg/mL) group.

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