Knockdown of KCNQ1OT1 Inhibits Proliferation, Invasion, and Drug Resistance by Regulating miR-129-5p-Mediated LARP1 in Osteosarcoma

Figure 4

KCNQ1OT1 promotes proliferation, invasion, and drug resistance by regulating miR-129-5p-mediated LARP1 in osteosarcoma cells. U20S cells were transfected with the control vector (control group), KCNQ1OT1-siRNA (siBCLAF1 group), KCNQ1OT1-siRNA+control inhibitor (siBCLAF1+inhibitor-NC group), or KCNQ1OT1-siRNA+miR-129-5p inhibitor (siKCNQ1OT1+miR-129-5p inhibitor group), respectively. (a) The protein expression of LARP1 in the control group, siBCLAF1 group, siBCLAF1+inhibitor-NC group, and siKCNQ1OT1+miR-129-5p-inhibitor group. (b) The cell proliferation in the control group, siBCLAF1 group, siBCLAF1+inhibitor-NC group and siKCNQ1OT1+miR-129-5p inhibitor group. (c) The percentage of cell invasion in the control group, siBCLAF1 group, siBCLAF1+inhibitor-NC group, and siKCNQ1OT1+miR-129-5p inhibitor group. (d) The protein expression of P-gp and MRP1 in the control group, siBCLAF1 group, siBCLAF1+inhibitor-NC group, and siKCNQ1OT1+miR-129-5p inhibitor group. (e) The IC50 for 5-Fu was calculated in the control group, siBCLAF1 group, siBCLAF1+inhibitor-NC group, and siKCNQ1OT1+miR-129-5p inhibitor group. Compared with the control group or siBCLAF1+inhibitor-NC group at . GAPDH was used as an invariant internal control for calculating protein fold changes.

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