EEF1D Promotes Glioma Proliferation, Migration, and Invasion through EMT and PI3K/Akt Pathway

Figure 3

Inhibition of EEF1D impaired proliferation, migration, and invasion of glioma cells. (a) Efficacy of EEF1D-siRNA on EEF1D expression in U87 and A172 glioma cells, respectively. (b) Quantification results of (a). (c) Effect of EEF1D on proliferation of U87 and A172 glioma cells in vitro. The U87 and A172 glioma cells were subjected to CCK-8 assay for 5 d. There was a significant difference between two groups at day 5. NC: negative control siRNA-transfected cells; siEEF1D: EEF1D-siRNA transfected cells ( as compared with NC). (d) Quantification results of (c). (e, g) Transwell assay and Boyden assay were performed to determine cell migration and invasion in glioma U87 and A172 cell transfected with EEF1D-siRNA or negative control siRNA, respectively. NC: negative control siRNA-transfected cells; siEEF1D: EEF1D-siRNA-transfected cells ( as compared with NC). Scale bars: 100 μm. (f, h) Quantification results of (c) and (e) respectively.

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