EEF1D Promotes Glioma Proliferation, Migration, and Invasion through EMT and PI3K/Akt Pathway

Figure 4

Downregulation of EEF1D could regulate the expression of EMT markers and suppressed the activation of the PI3K/AKT signaling pathway. (a) Protein levels of EMT markers were detected in U87 and A172 glioma cells by Western blot. GAPDH was also detected as the control of sample loading. NC: negative control siRNA-transfected cells. siEEF1D: EEF1D-siRNA-transfected cells. Data were based on at least three independent experiments and shown as the ( as compared with NC). (b) Quantification results of (a). (c) Protein levels of PI3K/Akt signal pathway were detected in U87 and A172 glioma cells by Western blot. GAPDH was also detected as the control of sample loading. NC: negative control siRNA-transfected cells; siEEF1D: EEF1D-siRNA-transfected cells. Data were based on at least three independent experiments and shown as the ( as compared with NC). (d) Quantification results of (c).

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