Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARδ-AMPK-PGC-1α Pathway in Dyslipidemic Conditions

Figure 7

Immunohistochemistry for macrophage antigen and AT1R and Oil Red O staining in frozen-sectioned aorta tissue slides from rabbits administered a cholesterol diet and aspirin. (a, b) The protein expressions for macrophage antigen and AT1R in aorta tissues were extremely increased in cholesterol diet group compared to control; however, they were decreased in aspirin-administered group. (c) The lipid accumulation in aorta tissues of cholesterol diet group was elevated compared to control; however, the accumulation was lowered in aspirin-administered group. Magnification is 200 times for images of immunohistochemistry and 40 times for images of Oil Red O staining. Densities for images were analyzed with the ImageJ program. The results of image density are expressed as (). Values were statistically analyzed by unpaired -test or one-way ANOVA. An upper line on the three bars means one-way ANOVA analysis. All experiments were repeated three and over times. Meaning of indications: C means rabbits fed with 1% cholesterol diet and CA means rabbits administered with 1% cholesterol diet plus aspirin of 100 mg/kg/day. .