Aspirin Improves Nonalcoholic Fatty Liver Disease and Atherosclerosis through Regulation of the PPARδ-AMPK-PGC-1α Pathway in Dyslipidemic Conditions

Figure 8

Immunohistochemistry for macrophage antigen and AT1R and Oil Red O staining in frozen-sectioned liver tissue slides from rabbits administered a cholesterol diet and aspirin. (a, b) The protein expressions for macrophage antigen and AT1R in liver tissues were increased in cholesterol-diet group compared to control; however, they were decreased in aspirin-administered group. (c) The lipid accumulation in liver tissues of cholesterol-diet group was elevated compared to control; however, the accumulation was lowered in aspirin-administered group. Magnification is 200 times. Densities for images were analyzed with the ImageJ program. The results of image density are expressed as (). Values were statistically analyzed by unpaired -test or one-way ANOVA. An upper line on the three bars means one-way ANOVA analysis. All experiments were repeated three and over times. Meaning of indications: Ctrl means normal control rabbits administered with normal, cholesterol means rabbits fed with 1% cholesterol diet, and cholesterol+aspirin means rabbits administered with 1% cholesterol diet plus aspirin of 100 mg/kg/day. , , .