Research Article
Study of the Relationship between Microbiome and Colorectal Cancer Susceptibility Using 16SrRNA Sequencing
Table 2
PCR amplification reaction system (20 μL).
| The reagents | Volume |
| 5 × PCR buffer | 4 μl | 2.5 mM dNTP | 2 μl | Template NDA | 10 ng | 5 μM primer | 0.8 μl | FastPfu polymerase | 0.4 μl | Distilled water | Up to 20 μl |
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