THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways

Figure 3

Effects of THAP11 silencing on GC cell growth, cell cycle, and apoptosis in vitro. (a, b) MKN-45 cells were transfected with si-THAP11 or control (NC-siRNA). Expression of THAP11 was detected by qRT-PCR at 24 h after transfection and by Western blot at 48 h after transfection. GAPDH was used as an internal control. (c) After transfection, MTT assay was used to detect the proliferation of MKN-45 cells at 24 h, 48 h and 72 h, respectively. (d) After transfection, cell number was counted at 24 h, 48 h, and 72 h, respectively. (e) At 24 h after transfection, cell cycle after was detected by flow cytometry. The graph shows the percentage of cells in each phase. (f) At 48 h after transfection, cell apoptosis was analyzed using the Annexin V-FITC apoptosis detection kit. The graph shows the early and late apoptosis of cells. Data are shown as . Compared with NC-siRNA, , Student’s -test.

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