THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways

<div>Effects of THAP11 overexpression on c-Myc expression and regulated c-Myc target genes. (a) MKN-45 cells were transfected with control or ov-THAP11, and c-Myc mRNA level was analyzed by qRT-PCR at 24 h after transfection. (b) Expression of c-Myc, cyclinD1, p27, and p21 protein was detected by Western blot at 48 h after transfection and the relative expression of proteins was quantitatively analyzed. (c) MKN-45 cells were transfected with NC-siRNA or si-THAP11, and c-Myc mRNA level was analyzed by qRT-PCR at 24 h after transfection. (d) Expression of c-Myc, cyclinD1, p27, and p21 protein was detected by Western blot at 48 h after transfection, and the relative expression of proteins was quantitatively analyzed. GAPDH was used as an internal control. 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411V388C96 378 102 374 102 310V104C102 38 97 33 29 26V0H246V26C187 32 181 36 181 104V339C211 365 250 390 290 390C357 390 381 345 381 276V109C381 40 374 32 315 26V0H524Z"></path></g><g transform="matrix(.013,0,0,-0.013,31.913,0)"><path d="M535 290V340H323V533H265V340H52V290H265V84H323V290H535ZM535 -22V28H52V-22H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,42.449,0)"><path d="M409 504C401 567 396 607 392 642C354 654 312 665 266 665C137 665 60 583 60 487C60 374 161 325 225 290C300 250 355 215 355 141C355 68 311 21 235 21C131 21 86 122 71 183L41 176C48 128 61 42 68 21C78 16 93 8 118 0C142 -7 175 -15 216 -15C349 -15 438 69 438 174C438 287 344 333 265 374C186 414 138 449 138 522C138 576 172 631 249 631C336 631 363 562 380 499L409 504Z"></path></g><g transform="matrix(.013,0,0,-0.013,48.455,0)"><path d="M517 162C503 123 484 88 467 68C445 42 417 34 341 34C291 34 256 34 237 47C219 59 213 81 213 128V317H308C395 317 402 311 415 240H444V431H415C403 364 398 356 307 356H213V584C213 613 215 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BioMed Research International

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Figure 4

Figure 4: THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways 