Hepatic Polarization Accelerated by Mechanical Compaction Involves HNF4α Activation

Figure 2

Mechanical compaction activates HNF4α P1 or P2 expression in HEK-293T cells or primary hepatocytes. (a, d) Relative luciferase activities of cells transfected with clones containing HNF4α P1 or P2, GAPDH, and NEG (one-way ANOVA analysis and Dunnett’s multiple comparison test, HNF4α P1 and P2, GAPDH vs. NEG, , ). (b, c, e, f) The relative luciferase activities of HNF4α P1 and P2 in HEK-293T cells or primary hepatocytes treated by centrifugation at 500-1500 ×  (two-way ANOVA analysis and Bonferroni posttests, 500-1500 g vs. 0 g, , , , ).