Hepatic Polarization Accelerated by Mechanical Compaction Involves HNF4α Activation

Figure 5

Disruption of F-actin polymerization partially but significantly reduced the relative luciferase activities of HNF4α P1 and P2 and the expression levels of HNF4α induced by mechanical compaction. (a) LatB effectively disrupted F-actin polymerization in subconfluent cell cultures. (b, c) The relative luciferase activities of HNF4α P1 and P2 and (d–f) the mRNA and protein levels of HNF4α in primary hepatocytes exposed to LatB and stimulated by 500 ×  were lower than those in cells stimulated by 500 ×  and untreated with LatB but still higher than those in cells just stimulated by 0 ×  (two-way ANOVA analysis and Bonferroni posttests, ^&LatB treated vs. 0 g, ^#LatB treated vs. 500 g, , , , ).

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