Hepatic Polarization Accelerated by Mechanical Compaction Involves HNF4α Activation

Figure 6

Disruption of E-cadherin-mediated cell adherent junctions partially but significantly reduced the relative luciferase activities of HNF4α P1 and P2 and the expression levels of HNF4α induced by mechanical compaction. (a) SHE78-7 effectively disrupted cell-cell contacts in subconfluent cell cultures. (b, c) The relative luciferase activities of HNF4α P1 and P2 and (d, f) the mRNA and protein levels of HNF4α in primary hepatocytes exposed to SHE78-7 and stimulated by 500 ×  were lower than those in cells stimulated by 500 ×  and untreated with SHE78-7 but still higher than those in cells just stimulated by 0 ×  (two-way ANOVA analysis and Bonferroni posttests, ^&SHE78-7 treated vs. 0 g, ^#SHE78-7 treated vs. 500 g, , , , ).

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