Inhibition of Wilms’ Tumor Proliferation and Invasion by Blocking TGF-β Receptor I in the TGF-β/Smad Signaling Pathway

Figure 2

TGF-β receptor I (TβRI) inhibition decreased epithelial-mesenchymal transition regulated by the TGF-β/Smad signaling pathway, resulting in Wilms’ tumor proliferation, migration, invasion, and apoptosis. (a) Cell counting kit-8 assay was performed to determine cell proliferation of G401 cells that were treated with different concentrations of TβRI inhibitor, TGF-β agonist, or DMSO for 4 h. (b) Wound healing assay was performed to determine the migration rate of G401 cells at 4 h and 24 h after treatment with DMSO, TβRI inhibitor, or TGF-β agonist (magnification, 20x). (c) Transwell-mediated invasion assay was performed to determine the invasion ability of G401 cells which were treated by DMSO, TβRI inhibitor, or TGF-β agonist. (d) Flow cytometry analysis was performed to determine the apoptosis rate of G401 cells. (e) Western blot was used to determine the expression of TGF-β1, P-smad2/3, E-cadherin, α-SMA, and MMP-7. GAPDH was used as a loading control. The relative expression was analyzed. (#, , , ).

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