Inhibition of Wilms’ Tumor Proliferation and Invasion by Blocking TGF-β Receptor I in the TGF-β/Smad Signaling Pathway

Figure 4

TGF-β receptor I (TβRI) inhibition reduces EMT in WT in vivo. (a) Hematoxylin-eosin staining in tumor treated with either TβRI kinase inhibitor or PBS (control) (magnification, 20 and 40). (b) Double-immunofluorescence showing that TβRI inhibition significantly decreased the expression of N-cadherin and β-catenin and considerably increased the expression of E-cadherin and cytokeratin (magnification, 40). (c) Western blot showing that the TβRI inhibitor significantly upregulate the expression of P-Smad2/3 and decreased the expression of Smad2/3. (d) Western blot of EMT relatively protein expression showed that treatment with the TβRI inhibitor significantly upregulated cytokeratin and E-cadherin and downregulated N-cadherin and MMP-7 (, ).

(a)

(b)

(c)

(d)