Research Article
HAND2-AS1 Works as a ceRNA of miR-3118 to Suppress Proliferation and Migration in Breast Cancer by Upregulating PHLPP2
Figure 4
PHLPP2 was a downstream target of miR-3118. (a) RNA pull-down assays were carried out to verify which mRNA could bind to miR-3118. (b) The binding sites between miR-3118 and PHLPP2 were predicted by bioinformatics. (c) PHLPP2 expression and protein were tested in cells transfected with miR-3118 inhibitor by RT-qPCR and western blot. (d) RIP assays were performed to certify that HAND2-AS1, miR-3118, and PHLPP2 coexisted in RNA-induced silencing complexes (RISCs). (e) Luciferase reporter assays were conducted to testify the competing relationship between HAND2-AS1 and PHLPP2.
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