Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer

Figure 1

Identification of the existence and stability of cfDNA in human BM. (a) cfDNAs isolated from BM (from donor 1) were detected by Qsep100 Bio-Fragment Analyzer (BiOptic). (b) cfRNAs extracted from fresh BM (from donor 1) were detected by Agilent 2100 Bioanalyzer. (c) The whole BM and BM supernatant from donors 1, 2, 3, 4, and 5 were incubated at room temperature for the indicated time; then, cfDNAs were extracted and detected quantitatively by qRT-PCR; normalized results were showed here. (d) Capillary electrophoresis detection of cfDNA isolated from the whole BM and BM supernatant (from donor 1) after incubation at room temperature for 7 days.

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