Potential of Using Cell-Free DNA and miRNA in Breast Milk to Screen Early Breast Cancer

Figure 3

BM cfDNA contains complete genetic information that is highly uniform with the whole genome. (a) Whole genome sequencing (WGS) libraries were generated using cfDNA from fresh and RT-7-days BM (donor 1), then detected by capillary electrophoresis. Results show that the major molecular weight size of three libraries is very similar. (b) WGS data of libraries in (a) were used to analyze copy numbers on a basic unit of 0.5 Mb (described in detail in Supplemental Text). Then, data visualization of copy numbers was plotted by using ggplot2 software. (c) The -score of each chromosome was calculated (described in detail in Supplemental Text) based on WGS data of cfDNAs from three fresh BM and three RT-7-days BM (donors 1, 2, and 3). The graphs show the statistical chromosomal -score of different samples.

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