Overexpression of NNT-AS1 Activates TGF-β Signaling to Decrease Tumor CD4 Lymphocyte Infiltration in Hepatocellular Carcinoma

Figure 4

NNT-AS1 expression levels in cells transiently transfected with NNT-AS1-specific siRNAs (siNNT) or control siRNA (siNC). (a) NNT-AS1 expression was determined by RT-qPCR after siRNA transfection of HepG2 cells (repeat three times). (b) RT-qPCR assays determined the mRNA levels of TGF-β, TGFBR1, and SMAD5 in HepG2 (repeat three times). (c) RT-qPCR assays determined the mRNA levels of TGF-β and SMAD5 in Huh7 (repeat three times). (d) Representative images of Western blotting that were used to determine the protein levels of TGF-β, TGFBR1, and SMAD5 in HepG2 and Huh7 cells. (e) The ImageJ software was used for semiquantitative analysis of the relative levels of TGF-β, TGFBR1, and SMAD5 based on the results from three Western blotting assays.

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