Toxicokinetics of 11 Gelsemium Alkaloids in Rats by UPLC-MS/MS

Table 3

Accuracy, precision, extraction efficiency, and matrix effects of 11 Gelsemium alkaloids in rat plasma ().

Compound	Concentration (ng/mL)	Accuracy (%)		CV (%)		Matrix effect (%)	Recovery (%)
		Intraday	Interday	Intraday	Interday
Humantenirine	0.1	103.2	98.4	5.7	9.9	95.4	84.0
	0.4	95.5	95.4	7.1	13.7	93.6	81.8
	18	99.4	99.2	3.0	7.5	97.3	89.9
	180	99.5	107.4	7.7	8.8	93.6	86.8
Humantenine	0.1	98.4	104.7	7.3	13.0	99.1	85.8
	0.4	97.7	98.3	2.1	5.2	98.8	89.1
	18	102.0	100.8	3.7	5.0	103.6	86.0
	180	96.5	106.3	4.7	8.4	98.1	90.0
Akuammidine	0.1	107.9	100.8	5.9	12.7	103.4	98.4
	0.4	107.3	101.7	8.0	6.7	94.1	95.4
	18	104.3	98.0	5.2	4.5	98.0	90.5
	180	96.3	99.7	5.0	4.4	107.2	98.3
Gelsevirine	0.1	106.5	109.2	5.7	12.3	98.5	89.7
	0.4	101.1	99.5	3.2	6.5	96.6	88.9
	18	99.3	95.6	5.8	9.6	99.5	93.2
	180	102.2	103.8	7.0	6.4	101.3	96.6
Rankinidine	0.1	99.3	101.5	14.9	13.3	103.2	93.8
	0.4	102.2	97.9	7.8	2.2	104.2	91.0
	18	103.2	105.0	8.8	12.6	107.7	86.5
	180	97.4	101.6	7.1	5.8	103.2	92.0
n-Methoxyanhydrovobasinediol	0.1	92.7	100.9	5.0	8.3	98.8	95.9
	0.4	106.0	97.9	4.0	4.5	97.7	89.0
	18	102.7	94.9	3.4	6.2	105.4	90.9
	180	97.4	103.8	2.5	4.0	103.4	90.6
Gelsenicine	0.1	104.5	98.2	10.9	13.8	90.6	91.6
	0.4	102.8	103.5	4.0	12.3	88.5	84.3
	18	105.1	97.0	3.3	5.3	95.1	82.2
	180	92.5	92.2	6.9	5.4	98.9	93.5
Gelsemine	0.1	86.9	96.7	16.7	12.1	97.2	82.5
	0.4	98.9	89.9	11.3	12.7	103.8	90.1
	18	106.5	91.2	13.7	8.8	107.8	86.0
	180	92.8	102.2	8.2	11.2	91.2	84.9
Koumine	0.1	113.2	91.8	6.9	15.9	94.2	95.6
	0.4	108.3	99.6	11.8	8.1	92.8	83.3
	18	99.6	91.7	8.3	11.2	91.6	82.5
	180	98.2	108.3	12.7	13.9	89.6	84.7
Koumidine	0.1	102.3	103.9	11.1	14.6	100.8	78.2
	0.4	105.5	101.2	9.5	6.1	97.7	79.8
	18	97.5	105.8	10.3	10.6	100.2	76.4
	180	99.1	96.6	5.7	8.9	94.0	82.7
Sempervirine	0.1	103.0	94.2	13.8	11.7	95.9	84.7
	0.4	96.5	104.3	6.4	8.8	97.9	77.4
	18	103.1	97.8	5.8	4.3	98.2	75.8
	180	101.0	96.7	9.7	4.3	106.0	85.6