Toxicokinetics of 11 Gelsemium Alkaloids in Rats by UPLC-MS/MS

Table 4

Stability of 11 Gelsemium alkaloids in rat plasma.

Compound	Concentration (ng/mL)	Autosampler ambient		Ambient 2 h		-20°C for 30 d		Freeze-thaw
		Accuracy (%)	CV (%)	Accuracy (%)	CV (%)	Accuracy (%)	CV (%)	Accuracy (%)	CV (%)
Humantenirine	0.4	104.2	8.5	102.1	4.0	107.9	11.7	103.0	10.5
	18	100.7	6.3	106.1	9.1	101.5	5.9	99.3	6.1
	180	98.0	5.0	106.8	7.8	99.9	5.7	97.0	8.9
Humantenine	0.4	94.5	5.3	98.8	7.6	107.3	12.6	99.8	11.6
	18	104.4	3.6	100.8	4.6	96.5	3.5	104.0	9.6
	180	99.2	3.5	106.5	4.5	100.9	1.4	105.8	3.7
Akuammidine	0.4	102.8	7.8	98.6	8.6	111.4	11.7	90.5	14.6
	18	97.6	6.7	109.7	5.9	103.5	9.6	96.8	9.4
	180	99.1	8.1	106.7	3.1	103.6	7.4	104.3	4.6
Gelsevirine	0.4	99.5	5.4	96.9	5.8	101.3	8.5	101.1	9.2
	18	101.9	1.9	99.3	2.1	94.8	10.6	106.1	9.6
	180	96.5	4.3	103.7	4.8	93.3	10.0	99.8	7.8
Rankinidine	0.4	99.5	7.8	90.3	9.1	89.7	8.5	87.4	11.7
	18	101.8	6.0	101.4	6.3	105.5	9.8	107.4	3.4
	180	96.6	6.9	106.5	5.1	104.7	7.9	101.6	5.7
n-Methoxyanhydrovobasinediol	0.4	96.0	8.3	94.4	6.1	102.5	4.6	92.8	9.7
	18	103.8	4.3	104.1	8.5	95.3	8.9	99.8	8.3
	180	99.3	6.7	95.0	7.2	98.9	5.2	108.9	8.3
Gelsenicine	0.4	92.4	11.7	106.6	5.3	100.8	7.3	92.1	7.4
	18	98.5	6.0	93.1	11.1	94.2	10.5	105.6	11.5
	180	105.0	9.8	103.8	12.0	92.0	5.3	104.5	13.3
Gelsemine	0.4	100.8	7.4	97.2	12.6	111.4	14.0	99.7	11.5
	18	96.6	3.1	104.3	11.1	104.2	13.2	110.7	8.5
	180	95.8	2.6	104.7	10.1	103.0	8.6	91.5	3.7
Koumine	0.4	100.6	10.4	112.5	12.1	108.1	13.0	105.8	14.7
	18	97.5	8.5	108.5	7.7	88.7	11.4	104.5	14.8
	180	102.3	8.7	93.7	4.7	94.1	5.4	92.5	7.4
Koumidine	0.4	98.6	7.5	97.2	6.2	97.5	14.8	108.1	13.0
	18	100.2	6.6	103.1	4.4	107.1	9.6	96.0	9.2
	180	104.8	5.5	101.5	6.6	92.8	3.5	101.4	7.8
Sempervirine	0.4	105.3	5.4	98.3	6.9	107.8	7.5	108.5	10.5
	18	99.9	5.3	99.7	2.9	100.5	10.8	95.4	8.3
	180	101.2	1.2	101.5	5.2	94.1	9.5	88.4	8.1