In Vitro Anticancer Potential of Berberis lycium Royle Extracts against Human Hepatocarcinoma (HepG2) Cells

Figure 4

Selective arrest of the cell cycle by BLE against HepG2 cells after 24 h of incubation. (a) The histograms were made by analysis on a ModFit LT model of analysis for control and BLE-treated HepG2 cells at mentioned concentrations for 24 h. BLE arrested the HepG2 cell’s cycle at the synthesis (S) phase of growth at 0.0625 mg/mL and G₁ phase after treatment at high concentrations of 0.5 and 1 mg/mL. (b) Gate 1 is plotted with FL2-W (width) and FL2-A (area) of the scattered cells, and gate 2 defines FSC-H (forward scatter) and SSC-H (side scatter) of the stained HepG2 cells.

(a)

(b)