Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings

Figure 5

Immunofluorescence staining analyzing HGF activity at various time intervals (6 h, 1 d, and 3 d). CLSM overlay of triple stain with DAPI (blue), cytoskeleton-actin stress fibers (red), and vinculin (green). The green fluorescent dots (white arrows) represent vinculin proteins. Three-dimensional microgroove samples showing overlay pictures (from top to bottom) for each microgroove type (Ti-MG, Ag-MG, and TiN-MG).