Pharmacokinetic Study of Thirteen Ingredients after the Oral Administration of Flos Chrysanthemi Extract in Rats by UPLC-MS/MS

Table 5

Stability of 13 analytes in rat plasma ().

Compounds	Spiked concentration (ng/mL)	Room temperature for 6 h		Three freeze-thaw cycles		Autosampler for 12 h		-80°C for 14 days
		Measured concentration (ng/mL)	RSD (%)	Measured concentration (ng/mL)	RSD (%)	Measured concentration (ng/mL)	RSD (%)	Measured concentration (ng/mL)	RSD (%)
Luteolin	20		1.5		3.0		1.9		1.7
	200		1.7		0.7		0.4		1.3
	4000		0.2		0.3		0.9		0.4
Cynaroside	20		4.4		4.4		1.0		3.8
	200		0.3		0.5		3.0		2.0
	4000		0.7		0.8		0.4		3.0
Luteolin 7-O-glucuronide	10		7.3		0.9		3.1		2.3
	100		1.1		0.4		0.4		0.3
	2000		0.8		0.4		2.0		1.2
Isochlorogenic acid C	5		3.8		1.7		8.7		1.8
	50		1.7		0.7		1.3		0.7
	1000		3.0		3.6		1.2		3.5
Cryptochlorogenic acid	5		1.9		1.7		4.0		3.8
	50		3.1		0.6		2.9		3.0
	1000		1.1		0.6		1.5		4.9
Chlorogenic acid	5		1.7		1.7		2.0		1.9
	50		4.6		0.7		1.6		0.7
	1000		0.8		0.6		1.3		0.2
Apigenin	5		2.0		1.8		3.7		1.9
	50		2.2		0.8		2.8		3.9
	1000		1.1		1.6		1.2		5.9
Acacetin	5		2.2		1.7		1.9		2.2
	50		1.6		11.4		1.5		1.4
	1000		0.8		0.5		0.5		5.9
Hyperoside	5		2.3		1.8		2.6		4.2
	50		3.9		3.9		3.3		3.6
	1000		2.0		0.4		0.3		1.9
Isoquercitrin	5		4.3		1.8		5.0		2.2
	50		2.4		1.5		1.2		4.1
	1000		2.0		2.0		2.0		3.0
Apigenin 7-glucoside	5		1.7		7.1		2.3		3.9
	50		2.0		1.1		1.6		0.8
	1000		1.2		0.6		0.9		2.3
Tilianin	1		9.1		10.0		11.1		11.1
	10		3.0		1.8		1.1		2.0
	200		0.1		0.1		0.7		0.5
Hesperidin	1		9.1		9.1		10.0		9.1
	10		3.9		1.0		1.1		2.1
	200		1.0		0.3		0.9		1.1