Sequence Analysis of the K13-Propeller Gene in Artemisinin Challenging Plasmodium falciparum Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study
Table 1
Total number of cases of IDT+ve, nested PCR+ve, PfKelch13 amplified, and 57 (89.06%) sequenced out of 64 P. falciparum isolates, by different sites and districts of Odisha, India.
SL no.
District
SL no. (area wise)
Sites
No. of samples
IDT Pf/Pv
PCR+ve Pf
K13 amplified (/%)
Samples sequenced
Samples collected from tribal areas (1-16)
All sequenced P. falciparum PfKelch13 isolates are of wild type, 89.06% (57 out of 64)
1
Keonjhar
1
Saria
2
2
2
2(100)
1
2
Kalahandi
2
Banigaon, Lanjigarh
35
35
23
19()
12
3
Kandhamal
3
Similipadar
31
30
22
18()
13
4
Kandhamal
4
Saperivatta
8
8
8
6()
3
5
Sundargarh
5
Sanajala, Baneigarh
1
1
1
1()
1
6
Sundargarh
6
Uparginia, Baneigarh
15
15
15
5()
4
7
Angul
7
Rugudidiha
1
1
1
1()
1
8
Angul
8
Bandhabhui
2
2
2
1()
1
9
Deogarh
9
Jalisuan, Barkot
5
3
2
1()
1
10
Raygada
10
Patalamba
12
12
8
7()
4
11
Raygada
11
Gartali
5
5
5
4()
3
12
Raygada
12
Kandsui
3
3
3
3()
2
13
Raygada
13
Parsali
2
2
2
2()
2
14
Ganjam
14
Uparabartala
5
4
1
1()
1
15
Malkangiri
15
Andrahal
2
2
2
2()
2
16
Malkangiri
16
Kanininga
1
1
1
1()
1
130
129
107
76(71)
52
Samples collected from urban areas CHC, PHC, and dist HQ hospital (17-24)
