Advanced Glycation End Products Induce Proliferation and Migration of Human Aortic Smooth Muscle Cells through PI3K/AKT Pathway

Figure 2

Effects of AGEs on HASMC proliferation and migration. (a) Optical density at 450 nm, measured in the CCK8 assay, following treatment with AGEs (5,10, 20, and 40 mg/L) for 24 h ( in each group). (b) Effect of AGEs on the expression of PCNA and Cyclin-D1 (PCNA and Cyclin-D1 are associated with cell proliferation, GAPDH was used as a loading control, in each group). (c) Migration of the HASMCs treated with AGEs (5,10, 20, and 40 mg/L) for 24 h ( in each group). (d) Effect of AGEs on the expression of MMP-9 and MMP-2 (MMP-9 and MMP-2 are associated with cell migration, GAPDH was used as a loading control, in each group). was defined as significant difference.

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