Biological Efficacy of Essential Oils and Plant Extracts of Cultivated and Wild Ecotypes of Origanum vulgare L.
Table 2
Antimicrobial activity of wild accessions of Origanum vulgare L. extract and essential oil against the bacterial strains tested based on the disc diffusion method and MIC value.
Antimicrobial activity
EO (10 μl/disc)
Standard antibiotic discs
EO
Standard drug (maxipime)
Acinetobacter baumannii-A8
18
18 mm (OFX)
13.78
31.25
Bacillus macerans-M58
28
19 mm (OFX)
31.25
15.62
Bacillus megaterium-M3
9 mm (SCF)
128
15.62
Bacillus subtilis-ATCC-6633
29
28 mm (SCF)
31.25
62.5
Bacillus subtilis-A57
28 mm (OFX)
62.5
125
Brucella abortus-A77
12 mm (OFX)
15.62
Burkholderia cepacia-A225
22 mm (SCF)
31.25
Clavibacter michiganensis-A227
35
25 mm (SCF)
15.62
31.25
Enterobacter cloacae-A135
20 mm (NET)
62.5
Enterococcus faecalis-ATCC-29122
10
18 mm (SCF)
62.5
125
Escherichia coli-A1
18
10 mm (SCF)
31.25
125
Klebsiella pneumoniae-A137
12 mm (OFX)
31.25
Proteus vulgaris-A161
12 mm (OFX)
15.62
Proteus vulgaris-Kukem-1329
12
13 mm (OFX)
62.5
125
Pseudomonas aeruginosa-ATCC-9027
13 mm (NET)
62.5
Pseudomonas aeruginosa-ATCC-27859
22 mm (NET)
31.25
Pseudomonas syringae pv. tomato-A35
22 mm (OFX)
62.5
Salmonella enteritidis-ATCC-13076
24 mm (SCF)
15.62
Staphylococcus aureus-A215
14
27 mm (SCF)
62.5
Staphylococcus aureus-ATCC-29213
22 mm (SCF)
31.25
Staphylococcus epidermis-A233
22 mm (SCF)
62.25
Streptococcus pyogenes-ATCC-176
12
10 mm (OFX)
62.5
Streptococcus pyogenes-Kukem-676
19
13 mm (OFX)
31.25
Xanthomonas campestris-A235
20 mm (SCF)
31.25
OFX = ofloxacin (10 μg/disc), SCF = sulbactam (30 μg) + cefoperazona (75 μg) (105 μg/disc), and NET = netilmicin (30 μg/disc) were used as positive reference standard antibiotic disc.
