ROS Plays a Role in the Neonatal Rat Intestinal Barrier Damages Induced by Hyperoxia

Figure 7

The protective effect of NAC on NCM460 cells treated with hyperoxia. (a) Specific bands for ZO-1, Occludin, and Claudin-4. (b) Densitometric analysis of Claudin-4, Occludin, and ZO-1. (c) Relative expression of ZO-1, Occludin, and Claudin-4 mRNA. Compared to the atmospheric air group, ZO-1, Occludin, and Claudin-4 expression levels significantly decreased in the hyperoxia group; however, after treatment with 20 mmol/l NAC, the levels of ZO-1, Occludin, and Claudin-4 gradually increased, and ZO-1 and Occludin came close to normal levels ( or in the hyperoxia group compared to the atmospheric air group, the air + NAC group, and the hyperoxia + NAC group; in the hyperoxia + NAC group compared to the atmospheric air group and the air + NAC group; in each group). β-Actin served as an internal control. Densitometric measurements were used to analyze ZO-1, Occludin, and Claudin-4 protein levels normalized to β-actin.

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