Research Article
Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49
Table 2
Design matrix of medium optimization for β-carotene production in CBS 277.49.
| Std. order | Variables | Results | C/N ratios | Cerulenin (μg/mL) | Ketoconazole (mg/L) | Observed values | Predicted values | β-Carotene (mg/L) | β-Carotene (mg/L) |
| 1 | 10 | 20 | 100 | 2.93 | 3.21 | 2 | 40 | 20 | 100 | 2.24 | 2.73 | 3 | 10 | 50 | 100 | 1.09 | 1.24 | 4 | 40 | 50 | 100 | 1.91 | 2.02 | 5 | 10 | 20 | 200 | 1.96 | 2.33 | 6 | 40 | 20 | 200 | 1.65 | 1.98 | 7 | 10 | 50 | 200 | 1.93 | 1.93 | 8 | 40 | 50 | 200 | 2.84 | 2.84 | 9 | 0 | 35 | 150 | 1.81 | 1.45 | 10 | 50 | 35 | 150 | 2.14 | 1.81 | 11 | 25 | 10 | 150 | 4.26 | 3.80 | 12 | 25 | 60.3 | 150 | 2.27 | 2.36 | 13 | 25 | 35 | 66 | 2.80 | 2.31 | 14 | 25 | 35 | 235 | 2.45 | 2.26 | 15 | 25 | 35 | 150 | 2.24 | 1.64 | 16 | 25 | 35 | 150 | 1.49 | 1.64 | 17 | 25 | 35 | 150 | 1.51 | 1.64 | 18 | 25 | 35 | 150 | 1.49 | 1.64 | 19 | 25 | 35 | 150 | 1.49 | 1.64 | 20 | 25 | 35 | 150 | 1.50 | 1.64 |
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