Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models

<div>Calculation of the optimal number of reference genes for quantitative analysis using geNorm. Pairwise variation (<span class="nowrap"><svg height="11.439pt" id="M153" style="vertical-align:-2.15067pt" version="1.1" viewbox="-0.0498162 -9.28833 46.9489 11.439" width="46.9489pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M687 650H462V622C543 612 549 605 530 547C498 447 422 252 372 126H370C302 298 229 492 204 563C188 607 191 615 262 622V650H17V622C77 616 93 608 122 534C180 389 262 172 329 -11H360C436 196 541 450 568 516C606 605 619 614 687 622V650Z"></path></g><g transform="matrix(.013,0,0,-0.013,8.017,0)"><path d="M524 0V26C466 32 460 36 460 104V297C460 393 411 449 331 449C302 449 276 437 248 419C223 402 201 387 181 372V451C137 432 90 420 42 411V388C96 378 102 374 102 310V104C102 38 97 33 29 26V0H246V26C187 32 181 36 181 104V339C211 365 250 390 290 390C357 390 381 345 381 276V109C381 40 374 32 315 26V0H524Z"></path></g><g transform="matrix(.013,0,0,-0.013,15.189,0)"><path d="M368 703H309L44 -163H104L368 703Z"></path></g><g transform="matrix(.013,0,0,-0.013,20.55,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,29.982,0)"><path d="M535 230V280H323V490H265V280H52V230H265V-3H323V230H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.518,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>)</span> of reference genes analyzed in different treatments was listed in (a, b) set the cut-off threshold value 0.15, calculating the optimal number of reference genes for precise quantitative in this work. WT, E100 (E200, E400), H100 (H200, H400, H800), A2.5 (A5, A10), and C0.05% (C0.1%, C0.2%, C0.4%), respectively, were the abbreviation for the control group, EtOH-100 mM (200 mM, 400 mM), H<sub>2</sub>O<sub>2</sub>-100 <i>μ</i>M (200 <i>μ</i>M, 400 <i>μ</i>M, 800 <i>μ</i>M), APAP-2.5 mM (5 mM, 10 mM, 20 mM), CCl<sub>4</sub>-0.05% (0.1%, 0.2%, 0.4%).</div>

BioMed Research International

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Figure 3

Figure 3: Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models 