BioMed Research International

Research Article

Selection of Suitable Reference Genes for qPCR Gene Expression Analysis of HepG2 and L02 in Four Different Liver Cell Injured Models

Table 2

Expression stability values of ten candidate reference genes in HepG2 analyzed by NormFinder.
RankWTEtOH
100 mM		EtOH
200 mM		EtOH
400 mM		H2O2
200 μM		H2O2
400 μM		H2O2
800 μM		APAP
2.5 mM		APAP
5 mM		APAP
10 mM		CCl4
0.1%		CCl4
0.2%		CCl4
0.4%
1HPRT1
0.011		HPRT1
0.004		CYC1
0.007		HPRT1
0.008		B2M
0.018		CYC1
0.013		TBP
0.011		HPRT1
0.013		GUSB
0.004		HPRT1
0.012		TBP
0.015		TBP
0.011		HPRT1
0.005
2TUBB2a
0.012		TBP
0.005		TBP
0.009		TUBB2a
0.013		TUBB2a
0.019		TUBB2a
0.017		GAPDH
0.011		GUSB
0.015		CYC1
0.010		B2M
0.018		CYC1
0.017		ACTB
0.013		CYC1
0.008
3CYC1
0.013		TUBB2a
0.005		SDHA
0.011		B2M
0.014		CYC1
0.020		TBP
0.019		SDHA
0.012		SDHA
0.018		TUBB2a
0.014		SDHA
0.019		SDHA
0.020		B2M
0.021		GUSB
0.009
4B2M
0.017		YWHAZ
0.012		B2M
0.011		TBP
0.016		TBP
0.027		HPRT1
0.024		HPRT1
0.015		YWHAZ
0.022		ACTB
0.020		TUBB2a
0.020		HPRT1
0.021		TUBB2a
0.021		TUBB2a
0.016
5TBP
0.018		SDHA
0.013		HPRT1
0.013		SDHA
0.018		GUSB
0.030		GUSB
0.025		B2M
0.017		CYC1
0.023		HPRT1
0.021		CYC1
0.022		TUBB2a
0.024		CYC1
0.021		SDHA
0.019
6SDHA
0.019		ACTB
0.014		GAPDH
0.015		CYC1
0.020		SDHA
0.030		YWHAZ
0.027		TUBB2a
0.017		TBP
0.024		TBP
0.022		ACTB
0.024		GUSB
0.024		GUSB
0.022		TBP
0.024
7ACTB
0.020		CYC1
0.022		GUSB
0.015		ACTB
0.025		YWHAZ
0.037		ACTB
0.031		CYC1
0.017		ACTB
0.028		YWHAZ
0.025		GUSB
0.025		YWHAZ
0.028		YWHAZ
0.022		B2M
0.029
8GUSB
0.021		B2M
0.032		TUBB2a
0.016		GUSB
0.027		ACTB
0.039		B2M
0.037		ACTB
0.019		GAPDH
0.029		B2M
0.029		TBP
0.029		GAPDH
0.032		SDHA
0.024		GAPDH
0.031
9YWHAZ
0.023		GAPDH
0.041		ACTB
0.020		YWHAZ
0.028		HPRT1
0.046		SDHA
0.043		GUSB
0.022		TUBB2a
0.030		SDHA
0.031		GAPDH
0.035		B2M
0.034		HPRT1
0.029		ACTB
0.037
10GAPDH
0.029		GUSB
0.042		YWHAZ
0.023		GAPDH
0.041		GAPDH
0.061		GAPDH
0.048		YWHAZ
0.024		B2M
0.032		GAPDH
0.039		YWHAZ
0.037		ACTB
0.037		GAPDH
0.044		YWHAZ
0.039