Environmental Pollutant Benzo[a]pyrene Induces Recurrent Pregnancy Loss through Promoting Apoptosis and Suppressing Migration of Extravillous Trophoblast

Figure 2

BPDE promotes apoptosis of Swan 71 cell through activating Bax and Caspase-3 while reducing Bcl-2 expressions. (a) Swan71 cells were exposed to 0, 0.25, 0.5, 0.75, or 1.00 μM BPDE for 24 h, and the apoptosis was measured by flow cytometry. (b) The quantitative statistics of FITC+ apoptosis rates. or indicates significant difference from the control cells. (c) The protein levels of Bax and Caspase-3 in Swan 71 cell treated by different concentration of BPDE were measured by Western blotting. (d) The quantitative statistics of scale value, representing relative protein level, determined by ImageJ. or indicates significant difference from the control cells.

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