Resveratrol’s Impact on Vascular Smooth Muscle Cells Hyporeactivity: The Role of Rho-Kinase Inhibition

Table 5

Values of EC 50 and E_max in the control and test groups: resveratrol + HA-1077 in concentrations of 20 μM/l and 100 μM/l.

	n^1	EC50 (M/l)	Emax (%)^2
Control (Res−)	8	4.53 ± 1.2 × 10^−8	100.0	—
HA-1077 (20 µM/l) (Res−)	8	1.49 ± 0.34 × 10^−7	87.0 ± 3.0
HA-1077 (20 µM/l) + resveratrol	12	2.49 ± 0.81 × 10^−7	76.4 ± 4.0
HA-1077 (100 µM/l) + resveratrol	10	5.02 ± 0.73 × 10^−7	70 ± 5.4	ns^c

¹The number of CRC used for the calculation; ²E_max calculated as a % of tissue maximal response to PHE; ^a value calculated value relative to control (Res−); ^b value calculated against HA-1077 (20 μM/l) (Res−); ^c value calculated against HA-1077 (20 μM/l) (Res+). The calculated values of the antagonist concentrations corresponding to 50% of the maximum effect are summarized in the table above. In the presence of HA-1077, they were 1.49 ± 0.34 × 10–7 M/l, 2.49 ± 0.81 × 10–7 M/l, and 5.02 ± 0.73 × 10–7 M/l. Significant EC50 differences were observed between the control group (Res−) and HA-1077 (Res−) at a concentration of 20 μM/l-4.53 ± 1.2 × 10–8 M/l. 1.49 ± 0.34 × 10–7 M/l (). All data are means ± SD.