Research Article

Genotyping of Clostridium perfringens Isolates from Domestic Livestock in Saudi Arabia

Table 1

Primers used for amplification of the genes responsible of producing specific toxins and the expected amplicon size together with the annealing temperature for the PCR.

GenePrimer sequence 5 to 3Amplicon size (bp)Annealing temp (°C)

Plc, cpa (α-toxin)CPA5L: AGTCTACGCTTGGGATGGAA
CPA5R: TTTCCTGGGTTGTCCATTTC
90055

cpb (β-toxin)CPBL: TCCTTTCTTGAGGGAGGATAAA
CPBR: TGAACCTCCTATTTTGTATCCCA
61255

etx (ε-toxin)CPETXL: GGGGAACCCTCAGTAGTTTCA
CPETXR: ACCAGCTGGATTTGAGTTTAATG
39655

ipa (ι-toxin)CPIL: AAACGCATTAAAGCTCACACC
CPIR: CTGCATACCCTGGAATGGCT
29355

Cpe (CPE)CPEF: GGAGATGGTTGGATATTAGG
CPER: GGACCAGCAGTTGTAGATA
23356

cpb2 (β2-toxin)CPBL: CAAGCAATTGGGGGAGTTTA
CPBR: GCAGAATCAGGATTTTGACCA
20055

NetB toxinAKP78: GCTGGTGCTGGAATAAATGC
AKP79: TCGCCATTGAGTAGTTTCCC
56055-58