Adaptive Evolution of Feline Coronavirus Genes Based on Selection Analysis

Table 1

Primers for amplifying partial S, nsp 12, nsp 13, nsp 14, and N genes.

Primer	Amplified gene	Sequence (5→3)	Product size (bp)	Annealing temperature (°C)
S2B2-F	S	TTACAGCAGATGTTTTAAGG	1623	52
S2B2-R		CAGATTCAGGTTAAACCGGTTG
SC1-F	S	TTGCGGTTTGTAATACTGGTG	1726	50
SC1-R		ACTTATGTAAAATGGCCTTGG
1b3-F	nsp12-13	GAGTGGATTCAAACACCTGTAAC	1394	56.7
1b1-R		GTCAACCAAGAGAAGTACAATACCA
1b6-F	nsp13-14	CTGTCAACCCTGAGTCAAAGC	1518	62.4
1b6-R		GGAATTGTAACGTAGACATGTACC
N1	N	CCAAAAGACGTGATCGTTCTAA	664	50
N2		GTTTTCTTCCAGGTGTGTTTGT