Evaluation of the Role of Human DNAJAs in the Response to Cytotoxic Chemotherapeutic Agents in a Yeast Model System

Figure 1

Rescue of the growth phenotype of ydj1Δ by the human DNAJAs. (a). Sequence comparison indicates that YDJ1 is more closely related to DNAJA1 and DNAJA 2, as indicated by the phylogenetic tree. The comparison included 49 human HSP40 sequences obtained from NCBI protein database. Comparison was performed using the Constraint-based Multiple Alignment Tool from NCBI (COBALT). (b). The growth of ydj1Δ complemented strains was evaluated by growing them in the absence of stressors, as described in the Materials and Methods section. The strains tested are as follows: YDJ1 (open squares), ydj1Δ (closed circles), ydj1Δ-DNAJA1 (open triangles), ydj1Δ-DNAJA2 (open rhombus), ydj1Δ-DNAJA3 (--X--), and ydj1Δ-DNAJA4 (open circles). Growth was monitored at specified intervals by measuring and aliquot of the culture at OD₆₀₀. The fold increase, relative to the initial OD₆₀₀ of the culture, is presented.

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