Evaluation of the Role of Human DNAJAs in the Response to Cytotoxic Chemotherapeutic Agents in a Yeast Model System

Figure 5

Sensitivity of the DNAJAs-expressing strains to menadione. (a) DNAJA1- and DNAJA2-expressing strains showed the most resistance to doxorubicin and cisplatin and were therefore exposed to 6.6 mM menadione (1 h at 30°C) to determine survival. Cells were washed with sterile water and serial dilutions (1 : 10–1 : 10⁵) of the treated cultures were spotted onto Leu⁻ + glucose plates. Growth was scored after 3 days of incubation at 30°C. The serial dilutions of the strains are shown. N-acetylcysteine (NAc, 20 mM) was added as cotreatment with menadione to the indicated strains. (b) Quantification of the survival of the tested strains. Survival was determined by counting the number of colonies in the respective dilutions and calculated on the basis of the growth of strains not treated with menadione. At least three sets of experiments were used in the statistical analysis. Average survival plus standard deviation is shown. Dil: serial dilutions. Significantly different.

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(b)