Evaluation of the Role of Human DNAJAs in the Response to Cytotoxic Chemotherapeutic Agents in a Yeast Model System

Figure 9

Effect of the expression of DNAJA3 and DNAJA4 in cells with wild type YDJ1 exposed to heat shock. (a) The survival to heat shock of the DNAJA3- and DNAJA4-expressing wild type cells containing was determined. Serial dilutions of the cells were plated onto Leu⁻ + glucose plates and incubated at 30°C (untreated controls) and at 37°C (heat shock). Growth was scored at 72 hours. WT is the positive control, resistant to heat shock. (b) Survival was determined by growth of the heat-shocked strain relative to the growth of nonheat-shocked cells. At least three sets of experiments were used in the statistical analysis. Average survival plus standard deviation is shown. Dil: serial dilutions; Doxo, doxorubicin.

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