Evaluation of the Role of Human DNAJAs in the Response to Cytotoxic Chemotherapeutic Agents in a Yeast Model System

Table 1

Yeast strains used in this study.

Strain	Genotype	Description
Wild type (WT)	MATa his3-1 leu2Δ met15Δ ura3Δ [pYX142 LEU2]	Parental S. cerevisiae strain (BY4741)
ydj1Δ	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142 LEU2]	ydj1 deletion strain
YDJ1	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142-YDJ1 LEU2]	ydj1 deletion strain complemented with wild type YDJ1 gene
ydj1-DNAJA1	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX1423-ydj1-DNAJA1 LEU2]	ydj1 deletion strain complemented with human DNAJA1 gene
ydj1-DNAJA2	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142-ydj1-DNAJA2 LEU2]	ydj1 deletion strain complemented with human DNAJA2 gene
ydj1-DNAJA3	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142-ydj1-DNAJA3 LEU2]	ydj1 deletion strain complemented with human DNAJA3 gene
ydj1-DNAJA4	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142-ydj1-DNAJA4 LEU2]	ydj1 deletion strain complemented with human DNAJA4 gene
rad52	MATa his3-1 leu2Δ met15Δ ura3Δ rad52Δ [pYX142 LEU2]	rad52 deletion strain
WT + DNAJA3	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142- DNAJA3 LEU2]	Wild type strain expressing the human DNAJA3 gene
WT + DNAJA4	MATa his3-1 leu2Δ met15Δ ura3Δ ydj1Δ [pYX142- DNAJA4 LEU2]	Wild type strain expressing the human DNAJA4 gene