Research Article

Increased Temperature Facilitates Adeno-Associated Virus Vector Transduction of Colorectal Cancer Cell Lines in a Manner Dependent on Heat Shock Protein Signature

Figure 1

Schematic methodology of rAAV transduction at hyperthermia. The key points of the experiment were marked and described on the timeline. The rAAV vector in the dose of 4 × 104 gc was added on the second day of the experiment. The CRC cells and colon fibroblasts were transduced for 48 h, then the media were replaced. The transduction efficiency was performed on seventh day of the experiment by GFP+ cells counting and qPCR method.