Increased Temperature Facilitates Adeno-Associated Virus Vector Transduction of Colorectal Cancer Cell Lines in a Manner Dependent on Heat Shock Protein Signature

Figure 5

Transduction efficiency of CCD-18Co cells. The studied cells were transduced with the rAAV/DJ vector at hyperthermia conditions (as described at M&M panel). The GFP (Green Fluorescent Protein) positive (+) cells were measured by the Countess II FL Automated Cell Counter and the qPCR method (panel (a)), flow cytometry (panel (b)), and visualized with an inverted fluorescence microscope (panel (c)). For panel (b) the Q1 and Q2 quadrants represents death cells (7-AAD positive staining). The Q3 and Q4 quadrants represents live cells (7-AAD negative staining). The GFP+ cells are located in Q2 and Q4 quadrants. Images of non transduced (BF) cells and transduced cells (FITC) were performed at 10x magnification (panel (c)). Statistically significant differences were analyzed between cells transduced at 37°C and cells transduced at hyperthermia conditions. Statistical significance for results of GFP+ cells were marked as asterisks () and for results of rAAV genome copies (gc) were marked as carets (^).

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