Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB Signaling Pathway

Figure 4

HRS activates autophagy in LPS induced ALI and inhibition of autophagy reverses the protective effect of HRS in LPS induced ALI. (a) and (b) Western blotting was used to observe the expression of LC3-II, P62, and Beclin-1 protein. (c) Hematoxylin-eosin staining (Scale bar = 50 μm). (d) ELISA assay was used to detected inflammatory factor levels. (e) Flow cytometry was used to detect the survival rate of HPMECs. (f) Flow cytometry was used to detect the apoptosis rate of HPMECs. All experiments of cells were repeated three times. Data were expressed as mean ± SD. Statistically significant differences: . LPS: lipopolysaccharide; HRS: Hydrogen-rich saline. CQ: chloroquine, an autophagy inhibitor; 3MA: 3-methyladenine, an autophagy inhibitor. All data were analyzed by one‐way analysis of variance (ANOVA) with Tukey’s multiple comparison post hoc test.

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