Citron Rho-Interacting Serine/Threonine Kinase Promotes HIF1a-CypA Signaling and Growth of Human Pancreatic Adenocarcinoma

Figure 1

CIT is overexpressed in human PDAC and predicts poor survival. (a) CIT mRNA is overexpressed in PDAC cells. qPCR was performed to analyze the expression of CIT in five PDAC cell lines (Capan-1, BxPC-3, AsPC-1, MiaPaCa II, and PANC-1) and one normal human pancreatic duct epithelial cell line (H6C7). , vs. H6C7. (b) CIT protein level is overexpressed in PDAC cells. Western blot was performed to analyze the expression of CIT in five PDAC cell lines (Capan-1, BxPC-3, AsPC-1, MiaPaCa II, and PANC-1) and one normal human pancreatic duct epithelial cell line (H6C7). (c) CIT expression is overexpressed in PDAC tissues. The expression of CIT in PDAC was analyzed using the data from the TGCA database with the tool GEPIA (http://gepia.cancer-pku.cn). 171 normal tissues and 179 PDAC tissues were involved in the analysis. . (d) CIT high expression predicts poor overall survival. The patients were cut into CIT high expression (n = 89) and CIT low expression (n = 89) groups by the median expression value. Log-rank (Mantel-Cox) test was applied for survival analysis. (e) CIT high expression predicts poor disease-free survival. Log-rank (Mantel-Cox) test was applied for survival analysis.

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