Citron Rho-Interacting Serine/Threonine Kinase Promotes HIF1a-CypA Signaling and Growth of Human Pancreatic Adenocarcinoma

Figure 3

CIT regulates apoptosis and cell cycle of PDAC cells. (a) CIT knockdown induces apoptosis in PDAC cells. Capan-1 and BxPC-3 cells were infected with lentivirus carrying sh-Ctrl or sh-CIT for 48 hours, then apoptosis of the cells was monitored. vs. sh-Ctrl. (b) CIT knockdown activates apoptotic activators in PDAC cells. Capan-1 and BxPC-3 cells were infected with lentivirus carrying sh-Ctrl or sh-CIT for 24 hours, and then western blot was applied to analyze apoptotic effectors. (c) CIT overexpression represses olaparib-induced apoptosis. Capan-1 with/without CIT overexpression was treated with olaparib (1 μM) for 24 hours, then apoptosis of the cells was monitored. . (d) CIT knockdown induces cell cycle arrest. Capan-1 cells were infected with lentivirus carrying sh-Ctrl or sh-CIT for 48 hours, and then the cell cycle of the cells was monitored with flow cytometry. , vs. sh-Ctrl. (e) CIT knockdown reduces Ki67 expression. Capan-1 cells were infected with lentivirus carrying sh-Ctrl or sh-CIT for 48 hours, and then Ki67 expression of the cells was monitored with flow cytometry. vs. sh-Ctrl.

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