Bioinformatical Analysis of miRNA-mRNA Interaction Network Underlying Macrophage Aging and Cholesterol-Responsive Difference between Young and Aged Macrophages

Figure 3

Cholesterol-responsive differentially expressed miRNAs and mRNAs between aged and young macrophages. In young macrophages, 6 miRNAs were differentially expressed in response to (oxLDL) (a) and 8 miRNAs in response to (acLDL) (b). Altogether, only miR-714 was downregulated in response to both acLDL and oxLDL. In aged macrophages, no differentially expressed miRNA was identified in response to oxLDL, and miR-5129 was the only differentially upregulated miRNA in response to acLDL (c). Therefore, miR-714 was the differentially expressed miRNAs between young and aged macrophage’s response to cholesterol. With regards to differentially expressed mRNAs, 47 were detected when treated with oxLDL (d) and 39 with acLDL (e) in young macrophages and 25 mRNAs expressed differentially in response to both oxLDL and acLDL (f). In aged macrophages, 30 and 16 mRNAs expressed differentially in response to oxLDL (g) and acLDL (h), respectively, and 13 mRNAs in response to both (i).

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