[Retracted] TRPM7 Upregulate the Activity of SMAD1 through PLC Signaling Way to Promote Osteogenesis of hBMSCs

Figure 1

The effects of TRPM7 on the expression of osteogenic markers. (a) The expression of TRPM7 mRNA in cells induced for 0, 3, 7, and 14 days. (b) (A) The expression of TRPM7 protein in cells induced for 0, 3, 7, and 14 days. (B) Relative expression levels of TRPM7 protein were quantified and plotted. (c) (A) The expression of TRPM7 protein in cells noninduced for 0, 3, 7, and 14 days. (B) Relative expression levels of TRPM7 protein were quantified and plotted. (d) The expression of TRPM7 mRNA in cells treated with TRPM7 shRNA and control shRNA induced for 14 days. (e) The expression of TRPM7 protein in cells treated with TRPM7 shRNA and control shRNA induced for 14 days. The relative expression levels of TRPM7 protein were quantified and plotted. (f) The expression of TRPM7 mRNA in cells induced with the osteogenic medium or the osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. (g) (A) Western blot analysis of TRPM7 protein in cells induced with the osteogenic medium or the osteogenic medium supplemented with Naltriben or 2-APB. (B) The relative expression levels of TRPM7 protein were quantified and plotted. (h) The expression of osteogenic markers ALP, COL1A1, RUNX2, OPN, and OCN in cells treated with TRPM7 shRNA and control shRNA induced for 14 days. (i) (A)Western blot analysis of osteogenic marker proteins ALP, COL1A1, and RUNX2 in cells treated with TRPM7 shRNA and control shRNA. (B) Relative protein expression levels of osteogenic markers were quantified and plotted. (j) The expression of osteogenic markers ALP, COL1A1, RUNX2, OPN, and OCN in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. (k) (A) Western blot analysis of osteogenic marker proteins ALP, COL1A1, and RUNX2 in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB. (B) Relative protein expression levels of osteogenic markers were quantified and plotted. TRPM7 shRNA: hBMSCs transfected with TRPM7-shRNA-vector; control shRNA: hBMSCs transfected with negative control-shRNA-vector; , , and .

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