[Retracted] TRPM7 Upregulate the Activity of SMAD1 through PLC Signaling Way to Promote Osteogenesis of hBMSCs

Figure 2

The effects of TRPM7 on the osteogenic differentiation in hBMSCs. (a) Analysis of ALP staining in cells treated with control shRNA and TRPM7 shRNA induced for 14 days. (b) Analysis of ALP activity (the unit for p-nitrophenyl: μmol) in cells treated with control shRNA and TRPM7 shRNA induced for 14 days. (c) Analysis of ARS staining in cells treated with control shRNA and TRPM7 shRNA induced for 14 days. (d) A quantification of ARS in cells treated with control shRNA and TRPM7 shRNA induced for 14 days. (e) Analysis of ALP staining in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. (f) Analysis of ALP activity (the unit for p-nitrophenyl: μmol) in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. (g) Analysis of ARS staining in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. (h) A quantification of ARS in cells induced with osteogenic medium or osteogenic medium supplemented with Naltriben or 2-APB induced for 14 days. Scale: 200 μm. , , and .

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